Abstract
Photoacoustic microscopy (PAM) is a scalable bioimaging modality; one can choose low acoustic resolution with deep penetration depth or high optical resolution with shallow imaging depth. High spatial resolution and deep penetration depth is rather difficult to achieve using a single system. Here we report a switchable acoustic resolution and optical resolution photoacoustic microscopy (AR-OR-PAM) system in a single imaging system capable of both high resolution and low resolution on the same sample. Lateral resolution of 4.2 µm (with ~1.4 mm imaging depth) and lateral resolution of 45 μm (with ~7.6 mm imaging depth) was successfully demonstrated using a switchable system. In vivo blood vasculature imaging was also performed for its biological application.
Highlights
Photoacoustic microscopy (PAM) is an emerging hybrid in vivo imaging modality, combining optics and ultrasound, which can provide penetration beyond the optical diffusion limit with high resolution
The diverted beam passed through another right angle prism, RAP2 (PS915H-A, Tholabs), and a variable neutral density filter, NDF2 (NDC-50C-4M, Thorlabs), and coupled on to a multimode fiber, MMF (M29L01, Thorlabs) using a combination of objective (M-10X, Newport, Irvine, CA, USA) and XY translator (CXY1, Thorlabs), which acts as the fiber coupler (FC)
The nanoparticle was scanned with a step size of 0.5 microns in order to find the resolution of the optical resolution photoacoustic microscopy (OR-PAM) system
Summary
Photoacoustic microscopy (PAM) is an emerging hybrid in vivo imaging modality, combining optics and ultrasound, which can provide penetration beyond the optical diffusion limit with high resolution. They have used two separate lasers (high energy laser at 532 nm for the AR and a low energy high repetition rate laser at 570 nm for the OR), making the system inconvenient, expensive, and not suitable for applications including oxygen saturation measurements [29] In any of these techniques, AR-PAM was not having dark field illumination and there were strong photoacoustic signals from the tissue surface. In vivo blood vasculature imaging was performed on mouse ear for demonstrating its biological application
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