Abstract
To reveal the succession procedure of microbial community in hybrid biological reactor (HBR), the molecular biological techniques of polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE), cloning and sequencing and fluorescence in situ hybridization (FISH) were applied. PCR-DGGE results showed that the microbial community accumulated in both suspended-growth and attached-growth biomasses. Proteobacteria was found to be the dominant genera of bacteria in the sludge. Denitrifying bacteria was found accumulated in the biofilms. FISH results showed that there were more nitrifying bacteria in the suspended-growth biomass than the attached-growth biomass, but less denitrifying bacteria in the former biomass.
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