Performance and mechanism of azo dyes degradation and greenhouse gases reduction in single-chamber electroactive constructed wetland system

Abstract

A single-chamber microbial fuel cell-microbial electrolytic cell with a novel constructed wetland system was proposed for synergistic degradation of congo red and reduction in emissions of greenhouse gases. The closed-circuit system showed higher chemical oxygen demand and congo red removal efficiencies by 98 % and 96 % on average, respectively, than traditional constructed wetland. It could also significantly reduce the emissions of CH4 and N2O (about 52 % CO2-equivalents) by increasing the electron transfer. Microbial community analysis demonstrated that the progressive enrichment of dye-degrading microorganisms (Comamonas), electroactive bacteria (Tolumonas, Trichococcus) and denitrifying microorganisms (Dechloromonas) promoted pollutant removal and electron transfer. Based on gene abundance of xenobiotics biodegradation, the congo red biodegradation pathway was described as congo red → naphthalene and alcohols → CO2 and H2O. In summary, the single-chamber closed-circuit system could significantly improve the degradation of congo red and reduce the emissions of greenhouse gases by influencing electron transfer and microbial activity.