Abstract
We present a polymer-based optofluidic surface enhanced Raman scattering chip for biomolecule detection, serving as a disposable sensor choice with cost-effective production. The SERS substrate is fabricated by using industrial roll-to-roll UV-nanoimprinting equipment and integrated with adhesive-based polymeric microfluidics. The functioning of the SERS detection on-chip is confirmed and the effect of the polymer lid on the obtainable Raman spectra is analysed. Rhodamine 6G is used as a model analyte to demonstrate continuous flow measurements on a planar SERS substrate in a microchannel. The relation between the temporal response of the sensors and sample flow dynamics is studied with varied flow velocities, using SERS and fluorescence detection. The response time of the surface-dependent SERS signal is longer than the response time of the fluorescence signal of the bulk flow. This observation revealed the effect of convection on the temporal SERS responses at 25 µl/min to 1000 µl/min flow velocities. The diffusion of analyte molecules from the bulk concentration into the sensing surface induces about a 40-second lag time in the SERS detection. This lag time, and its rising trend with slower flow velocities, has to be taken into account in future trials of the optofluidic SERS sensor, with active analyte binding on the sensing surface.
Highlights
Optical biosensing is a diverse and evolving research field aiming for simplified and high-sensitivity diagnostic tools for the detection of biologicals/chemicals in environmental monitoring, medical analysis, food safety, and security
We present a polymer-based optofluidic surface enhanced Raman scattering chip for biomolecule detection, serving as a disposable sensor choice with cost-effective production
Label-free approaches are interesting since they do not require label molecules to be added, which could disturb the binding event, non- adsorb to the surface and complicate the process with extra cleaning steps. This has motivated the development of label-free optical sensor technologies, including interferometric, resonant-cavity, photonic crystal, surface plasmon resonance (SPR), and surface-enhanced Raman scattering (SERS) sensors [1]–[3]
Summary
The response time of the surface-dependent SERS signal is longer than the response time of the fluorescence signal of the bulk flow. This observation revealed the effect of convection on the temporal SERS responses at 25 μl/min to 1000 μl/min flow velocities. The diffusion of analyte molecules from the bulk concentration into the sensing surface induces about a 40-second lag time in the SERS detection This lag time, and its rising trend with slower flow velocities, has to be taken into account in future trials of the optofluidic SERS sensor, with active analyte binding on the sensing surface.
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