Performance and bacterial community of bio-electrochemical system treating simulated domestic wastewater containing low concentration of cephalosporin antibiotics

Abstract

ABSTRACT This study investigated the effects of five cephalosporin antibiotics (ceftazidime, ceftriaxone, cefdinir, cefixime and cefepime) on performance and bacterial community structure in bio-electrochemical systems (BES) and sequencing batch biofilm reactor (SBBR). The results showed that the external electric field had no significant effect on the removal of COD and ammonia nitrogen in water. The removal rates of five antibiotics in BES increased by 28.5%, 20.0%, 9.1%, 21.0%, and 11.5%, respectively. High-through sequencing showed that microbial membrane-growing process increased species diversity, and antibiotics had a significant inhibitory effect on the initial biofilm of the reactor. As time progressed, the inhibitory effect was weakened, and the microorganism were tolerated and re-enriched. The increase in the type and concentration of antibiotics and the applied electric field had a significant effect on the microorganisms in the reactor. The dominant microorganisms for antibiotic removal in the SBBR were Luteococcus, Cloacibacterium, Dysgonomonas, and Ottowia. The dominant bacteria in the BES were Ottowia and Tahibacte. The abundance of these strains increased significantly during antibiotic acclimation. The abundance of Ottowia, Tahibacter, and Nakamurella were significantly higher than SBBR. Thus the BES system had a good antibiotic degradation effect. The BES can effectively treat simulated domestic sewage containing multiple antibiotics, laying a theoretical foundation for the actual wastewater treatment.