Abstract
A report of the levels of Perfluorooctane Sulfonate (PFOS) and Perfluorooctanoic Acid (PFOA) in the fish from Lake Victoria is presented. Two fish species namelyLates niloticus (Nile perch) and Oreochromis niloticus (Nile tilapia) were obtained from Winam gulf of Lake Victoria, Kenya and analysed for PFOS and PFOA in muscles and liver using LC/MS/MS. Concentrations value of PFOS in Nile perch muscles of up to 10.50 and 35.70 ng/g for liver samples were obtained. Nile tilapia concentration values were of up to 12.40 and 23.70 ng/g for muscles and liver samples respectively. The accuracy and precision of the method were validated, and the effectiveness of the method in determining the contents of these two perfluorinated compounds in fish matrice was also demonstrated. The lowest limit of quantification (LOQ) was 0.5 ng/g and limit of detection was 0.05 ng/g. Typical values for precision obtained were 0.15 – 3.8% for HPLC/MS/MS, with concentrations ranging from 0.5 to 1000 ng/ml. Key words: PFOS, PFOA, LC/MS/MS, fish, Lake Victoria.
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