Perfil do corpo celular de neurônios mioentéricos nadh-diaforase positivos do estômago de ratos (Rattus norvegicus) submetidos ao alcoolismo crônico

Sonia Lucy Molinari,Paulo Alexandre Galvanini,Marli Aparecida Dos Santos Pereira,Marcílio Hubner De Miranda Neto,Maria Montserrat Diaz Pedrosa Furlan

doi:10.4025/actascibiolsci.v26i3.1599

Abstract

The purpose of this work was to compare the profile of the cell body of the NADH-diaphorase positive myenteric neurons from the stomach of rats. It was used 10 animals (Rattus norvegicus), from groups a) control (n = 5), that during 210 days had ad libitum supply of chow with normal protein level (22%) and water; and b) experimental (n = 5), that during 210 days had ad libitum supply of chow with normal protein level (22%) and sugar cane brandy diluted to 30 Gay Lussac (30o v/v). The stomachs collected and subjected to the technique for neuronal staining. The cell body of the neurons (n = 1.000) was measured through a computerized system of image analysis. The profile of the neurons from the control rats ranged from 60.16 and 638.64 µm2. In the experimental group the values ranged from 40.84 to 599.15 µm2. We observed a significant decrease on the cell body size, increase of the small neurons and decrease of the large neurons

Highlights

The myenteric plexus, an integral part of the enteric nervous system, has a fundamental role on the functions carried out by the gastrointestinal tract
As for the rat stomach, the ganglia of the myenteric plexus of the aglandular region are found between the longitudinal and circular smooth muscle layers of the outer muscle tunica; on the glandular region they are found embedded in the longitudinal layer of the muscle tunica (Oliveira et al, 2000)
It was used the stomach of 10 laboratory rodents, adult male Rattus norvegicus, albine, from the Wistar strain, supplied by the Central Biotery of the State University of Maringá, state of Paraná

Summary

Introduction

The myenteric plexus, an integral part of the enteric nervous system, has a fundamental role on the functions carried out by the gastrointestinal tract. This plexus has varied organization, location and constitution, when its phylogenesis (Irwin, 1931; Matsuo, 1934; Burnstock, 1959; Rash and Thomas, 1962; Bennett and Cobb, 1969; Gabella, 1979; Souza et al, 1982, 1988; Christensen et al, 1984; Timmermans et al, 1991; Molinari et al, 1994; Stabille et al, 1998) or its appearance in the organs of. It is observed a greater density of neurons next to the central region of the limiting fold than on the region next to the greater gastric curvature (Molinari et al, 2002); on the glandular region, the neuronal density is greater next to the lesser gastric curvature (Fregonesi et al, 1998; Oliveira et al, 2000)

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