Abstract
Abstract It is extremely difficult to remove artifacts common to eye tissue such as folds, shrinking raisin effect, and parched earth effect. By adjusting our fixation and processing procedures, we attempted to reduce or eliminate these artifacts. We tested the fixatives from various vendors to determine which of them is most compatible with eye tissue and which would eliminate artifacts. We found Pen-Fix (alcoholic formalin; Richard Allen, Richland, Ml) produced optimal fixation (well defined nuclear detail, without bubbling artifact). In similar testing, we found Peel-a-way (Shandon-Lipshaw, Pittsburgh, PA) to be the optimal paraffin for processing and embedding ocular tissues.
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