Abstract
Topical application of therapeutic agents has been a mainstay in Dermatology for the treatment of skin disorders but is not commonly used for systemic delivery. For a topically applied agent to reach distant body sites it must first overcome the barrier function of the skin and then penetrate into deeper structures before reaching the systemic circulation. This has limited the use of topically applied agents to those having specific charge, solubility and size restrictions. Pretreatment of the skin with ablative fractional laser appears to enhance the uptake of some topically applied drugs but the ability to effectively deliver agents to distant sites is largely unproven. In this report we used a fractional ablative Erb:YAG (Erbium/Yttrium Aluminum Garnet) laser to facilitate the transfer of bone marrow stem cells through the skin in a murine bone marrow transplant model. Chimerism could be detected in the peripheral blood of recipient C57BL/6 mice that were pretreated with ablative fractional laser and had topically applied enhanced green fluorescent protein (GFP) labeled bone marrow cells from syngeneic donor transgenic mice. This study indicates that fractional laser can be used to deliver stem cells through the skin and remain functionally intact.
Highlights
Stem cell based therapies have the potential to address many disorders that currently have few if any therapeutic alternatives [1,2,3]
In this report we describe the delivery of lineage negative (Lin2) bone marrow cells to the skin of irradiated mice using an ablative fractional laser with the goal of achieving functional bone marrow transplantation
In this report we have utilized a bone marrow transplantation model to demonstrate that fractional lasers can be used to assist in the percutaneous delivery of stem cells
Summary
Injection and surgical implantation techniques have been used when trying to administer cells locally to specific sites. Delivering cells directly to broad areas at predetermined depths is not possible with these techniques. We have examined the use of factional lasers to create micro-channels in the skin that may be used as conduits for cell delivery locally to tissues and allow for systemic administration [4,5,6]. In this report we describe the delivery of lineage negative (Lin2) bone marrow cells to the skin of irradiated mice using an ablative fractional laser with the goal of achieving functional bone marrow transplantation. We have demonstrated that stem cells can be delivered to the skin, become systemically distributed, engraft into distant organs and remain functionally intact. The cells were given at predetermined depths and could cover broader areas in a more uniform fashion
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