Abstract
Research about sepsis in animal model is interesting to get the right therapeutic method for human being. We did the research to study about the value of body temperature in mice’s various body area in order to get the important information if we are going to do the translational research in animal model about sepsis. One clinical signs of the sepsis mice model is changes in body temperature. An easy way to examine body temperature is using infrared non-contact thermometer. This study aimed to compare the body temperature using infrared non-contact thermometer at the abdomen and anal area. We used male mice, weighing 25–30 g, divided into two groups (control and treatment groups). The control group injected with NaCl 0.9% solution, with the amount of NaCl 0.9% volume equal to Lipopolysaccharide (LPS). In the treatment group injected with 2.5 mg/kg BW of LPS intraperitoneally. Body temperature was measured in abdomen (tabd) and ananl (tan) area at 8th and 24th hour after treatment. Body temperature value tabd was higher than tan. Lipopolysaccharide injection increase body temperature but was not significant when compared to the control group (8th and 24th hour). The mean difference between tabd and tan in 8th control groups were 2.12oC respectively. The mean difference between tabd and tan in 24th hour control groups 4.6oC. The mean difference in treatment groups (8th hour) was 4.66oC, while it was 4.77oC in the 24th groups. Giving 2.5 mg/kg BW LPS intraperitoneally did not rise the body temperature significantly as compare to control groups. But, body temperature at anus area using non-contact infrared thermometer after treatment showed lower results as compared to that of at abdomen significantly.
Highlights
Temperature measurement is an important indicator of infection in laboratory animal models
We tested the comparison of the value of body temperature measurement in anus and abdomen area using non-contact infra red thermometer in male mice
Mice in control group did not show any changes in activity at 1st, 8th, or 24th hour post-NaCl 0.9%-injection
Summary
Temperature measurement is an important indicator of infection in laboratory animal models. We can measure body temperature in the anus or abdomen. We tested the comparison of the value of body temperature measurement in anus and abdomen area using non-contact infra red thermometer in male mice. Rectal thermometer is the most commonly used device due to accuracy concern This technique of measurement has several weaknesses including: requirement of anesthesia in mice to avoid injury (Kawakami et al, 2018), the device is relatively expensive, and repeated measurements may cause death in mice (Suckow et al, 2001). Mice included in the order of rodentia, family muridae (Lundrigan et al, 2002) These mice have genetic and biological characteristics of mammals. At the end of the 19th and 20th centuries, these mice were farmed according to laboratory requirements based on skin color and genetic characteristics. The room temperature in laboratory is generally set to 20-22oC, to ensure the comfortability of the staffs (Speakman and Keijer, 2013)
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