PERBEDAAN EFEKTIVITAS CHLORHEXIDINE GLUKONAT 0,2% DENGAN TEH HIJAU (CAMELLIA SINENSIS) TERHADAP JUMLAH PORPHYROMONAS GINGIVALIS

Devi Dwipriastuti,R Rama Putranto,Welly Anggarani

doi:10.30659/odj.4.1.50-54

Abstract

Background: Periodontitis is a periodontal inﬂammation caused by plaque that contains pathogens, Porphyromonas gingivalis is one of them. Chlorhexidine therapy used to reduce the number of pathogenic bacteria causing periodontitis. Green tea contains polyphenols such as epigallocatechin-3-gallic as antibacterial agent that can kill the bacteria Porphyromonas gingivalis. This study aims to determine diﬀerences in the eﬀectiveness of chlorhexidine gluconate 0.2% with green tea (Camellia sinensis) various concentrations to decrease the amount of bacteria Porphyromonas gingivalis.Method: This research was experimental with post test only design. Method used in antibacterial test was a drop plate misra. The experimental group consisted of six groups: chlorhexidine gluconate 0.2% and green tea group with a concentration of 100%, 75%, 50%, 25% and 12.5%. minimum bactericidal concentrations againts Porphyromonas gingivalis was evidenced by counting the number of colonies that formed on agar. Analysis data was using One Way ANOVA continued by Post hoc tests Tamhane.Result: The results showed that chlorhexidine gluconate 0.2% with green tea eﬀective to decrease the amount of bacteria Porphyromonas gingivalis(ANOVA p <0.05). The results between the groups showed green tea 100%, 75% and 25%, have same eﬀect compared to chlorhexidine gluconate 0.2% in reducing Porphyromonas gingivalis.Conclusion: In this experiment showed that chlorhexidine gluconate 0.2% and green tea extract 100%, 75%, 50%, 25% and 12.5% were able to decrease the amount of Porphyromonas gingivalis.

Highlights

0.2%, Porphyromonas contains pathogens, Porphyromonas gingivalis is one of them
Result: The results showed that chlorhexidine gluconate 0.2% with green tea effective to decrease the amount of bacteria Porphyromonas gingivalis(ANOVA
25%, have same effect compared to chlorhexidine gluconate 0.2% in reducing. In this experiment showed that chlorhexidine gluconate 0.2% and green tea extract 100%, 75%, 50%, 25% and 12.5% were able to decrease the amount of Porphyromonas gingivalis

Summary

Conclusion

In this experiment showed that chlorhexidine gluconate 0.2% and green tea extract 100%, 75%, 50%, 25% and 12.5% were able to decrease the amount of Porphyromonas gingivalis. Periodontitis merupakan salah satu klasifikasi dari penyakit periodontal dimana terjadi respon host terhadap akumulasi plak yang melekat di permukaan gigi dan gingiva pada dentogingival junction yang mengandung berbagai mikroorganisme. Porphyromonas gingivalis adalah bakteri gram-negatif anaerob yang merupakan flora normal di dalam rongga mulut manusia yang banyak ditemukan pada area sulkus gingiva, plak subgingiva, lidah dan tonsil. Dwipriastuti/Putranto/Anggarani chlorhexidine sebagai obat kumur efektif digunakan sebagai anti plak dan juga antibakteri yang dapat mengurangi keparahan dari peradangan gingiva[3,4]. Chlorhexidine glukonat merupakan obat kumur yang sering digunakan pada masyarakat luas, chlorhexidine sendiri merupakan suatu zat antibakteri dengan sifat bakterisida dan efektif untuk bakteri gram positif dan gram negatif[5,6]. Penggunaan chlorhexidine sebagai antiseptik ternyata diketahui memiliki efek samping jika digunakan dalam jangka waktu yang lama, seperti pewarnaan pada gigi, adanya sensasi dan rasa yang tidak enak[2]. EGCG bekerja aktif dalam menekan terjadinya inflamasi, membunuh dan menghambat berbagai mikroorganisme dan memiliki kemampuan sebagai antioksidan[7,8,9]

METODE PENELITIAN

Hasil pengamatan jumlah koloni bakteri Porphyromonas gingivalis yang dihitung

DAFTAR PUSTAKA