Abstract
Water plants are the hydrophytic plants that occupy the highest ecology in aquatic environments. 
 Alternanthera reinickii and Ludwigia sp are freshwater ornamental plants that are in great deman d of 
 aquascape lovers market. Plant tissue culture is a technique to grow the growing parts of plants, 
 either in the form of cells, tissues or organs in aseptic conditions in vitro. The study was conducted on 
 15 January to 15 April 2018, at the Center for Biotechnology Research and Bogor Agricultural 
 Genetic Resources. This research is done by using experiment method in laboratory. From the results 
 of research on ornamental plants Alternanthera reinickii data obtained on the leaf paremeter giving 
 regeneration substances grow the type of BAP with a concentration of 0.3 ml / l capable of producing 
 the largest number of leaves. In shoot height parameters obtained the highest results using Kinetin 
 treatment concentration 0.1 ml / l. In the parameter of shoot num ber of growth regulator substances 
 BAP concentration of 0.5 ml / l resulted in the largest number of shoots. In the parameter of the root 
 number of BAP treatment with the concentration of 0.3 ml / l shows the largest number of roots. In 
 contrast to the ornamental plant Ludwigia sp BAP treatment of 0.1 ml / l concentration is able to 
 produce the highest number of leaves In the parameters of shoot BAP treatment at low concentrations 
 of 0.1 ml / l. on the parameters of BAP treatment buds concentration of 0.3 m l / l resulted in the 
 largest number of shoots. In the parameter of the root number of BAP treatme nt, the concentration of 
 0.1 ml/l showed the highest number of roots.
 Keywords: Alternanthera reinickii, Ludwigia sp, Tissue culture, BAP, Kinetin
Highlights
Tanaman air merupakan, tanaman hidrofit yang menempati ekologi tertinggi di dalam lingkungan perairan
the hydrophytic plants that occupy the highest ecology in aquatic environments
freshwater ornamental plants that are in great demand of aquascape lovers market
Summary
Tanaman hidrofit yang menempati ekologi tertinggi di dalam lingkungan perairan. Alternanthera reinickii dan Ludwigia sp adalah tanaman hias air tawar yang banyak diminati pasar pecinta aquascape. Sehingga perlu dilakukannya perbanyakan pada kedua jenis tanaman hias air tawar jenis Alternanthera reinickii dan Ludwigia sp. Teknik ini dicirikan oleh kondisi aseptik, penggunaan media kultur buatan dengan kandungan nutrisi lengkap dan penambahan zat pengatur tumbuh (ZPT). Zat pengatur tumbuh yang sering digunakan untuk menginduksi pertumbuhan adalah golongan Sitokinin dimana pada penelitian ini jenis yang digunakan adalah Kinetin dan BAP (Santoso dan Nursandi, 2003). Dalam kultur jaringan adalah BAP (6-benzylaminopurrine) yang merupakan salah satu Sitokinin sintetik yang aktif dan daya merangsangnya lebih lama karena tidak mudah dirombak oleh enzim di dalam tanaman (George dan Sherrington, 1984). Penelitian ini bertujuan untuk mencari jenis Sitokinin dan kosentrasi yang tepat terhadap multipikasi pertumbuhan tanaman hias air tawar Alternanthera reinickii dan Ludwigia sp secara in vitro
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