Abstract
<p><em>Th</em><em>e</em><em> research </em><em>wa</em><em>s done</em><em> to study the potential of ketepeng leaf meal and lemuru fish oil as agents to reduce methane production</em><em>. The composition of feed supplements, lamtoro leave meal (LLM), urea (U) and mollases (M) </em><em>were </em><em>in different ratios</em><em>:</em><em> 1:5, 1:6 and 1:7 and ketepeng</em><em> leaf</em><em> meal (DKM) and lemuru fish oil (MIL). The feed supplement was added to the native grass as a basal diet</em><em>,</em><em> P1: U: M = 1:5, DKM, P2: U: M = 1:5, MIL, P3: U: M = 1:6, DKM, P4: U: M = 1:6, MIL, P5: U: M = 1:7, DKM, P6: U: M = 1:7, MIL. </em><em>R</em><em>esearch </em><em>was </em><em>done with in vitro gas technique. </em><em>I</em><em>noculum </em><em>was taken from </em><em>rumen fluid </em><em>of</em><em> a </em><em>fistulated </em><em>male cow</em><em>,</em><em> </em><em>which was taken</em><em> before feed</em><em>ing</em><em> in the morning. </em><em>R</em><em>umen fluid pH </em><em>did not </em><em>significant</em><em>ly</em><em> different, </em><em>indicating</em><em> the microbial fermentation in rumen run well and normally </em><em>we</em><em>re not affected by the treatment. </em><em>The VFA</em><em> production</em><em> of</em><em> P5 and P6</em><em> was significantly lower than</em><em> P1, P2, P3 and P4 </em><em>(</em><em>P &lt;0.05). Production of C2 and C3</em><em> were</em><em> relatively the same except </em><em>for</em><em> P6 (P &lt;0.05) </em><em>which relative higher</em><em>. </em><em>Ratio of</em><em> C2/C3 and inversely related C3, </em><em>when </em><em>C3 production </em><em>increased the </em><em>C2/C3 ratio </em><em>decreased </em><em>(P &lt;0.05)</em><em>. M</em><em>ethane production </em><em>of</em><em> P6 </em><em>was higher but did not</em><em> different </em><em>with</em><em> P4 and P5. </em><em>It can be concluded that</em><em> diet </em><em>which </em><em>produced low methane </em><em>was</em><em> the native grass supplemented with urea and molases at ratio 1: 5 </em><em>contained either </em><em>ketepeng lea</em><em>f</em><em> meal </em><em>or</em><em> lemuru fish oil. </em><em></em></p><p><em><br /> <strong>Key</strong></em><strong><em> </em></strong><strong><em>words</em></strong><em>: native grass, supplement, ketepeng leaf meal, lemuru fish oil, methane production</em><em>.</em></p>
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