Abstract
This study aimed to compare the progesterone concentrations in Waringin sheep estrus cycle which induced by PGF2α or the combination of PGF2α and GnRH. This study used six Waringin sheep those were divided into twice groups, K1 and K2. K1(n=3) were injected by 7.5 mg PGF2α intramuscularly for 10 days, while K2 (n = 3) were injected by PGF2α and GnRH. On the first day K2 were injected by 7.5 mg PGF2α, then followed by injection 50 µg of GnRH on day 8th and re-injected by 7.5 mg PGF2α on day 15th. On day 18th, sample was re-injected using 50 µg GnRH. Waringin sheep those showed estrus symptoms were detected visually and with signs showed by sheep stud. Blood samples were taken on the 7th, 14th, and 21st day after the peak heat. The measurement of progesterone concentration was conducted by enzyme linked immunosorbent assay (ELISA). The results showed those the concentrations of progesterone on day 7th, 14th, and 21st on K1 vs K2 were 1.324±1.079, 7.607±8.922, and 5.220±1.653 vs 4.705±3.369, 4.184±5.512, and 1.797±0.898 ng/mL (P>0,05), respectively. In conclusion, the concentration of progesterone of Waringin sheep after inducing with PGF2α or combination PGF2α and GnRH at different cycle periode did not show differences. Keywords: Estrus Cycle, GnRH, PGF2α, Progesterone, Waringin Sheep
Highlights
This study aimed to compare the progesterone concentrations in Waringin sheep estrus cycle which induced by PGF2α or the combination of PGF2α and Gonadotrophin Releasing Hormone (GnRH)
The results showed those the concentrations of progesterone on day 7th, 14th, and 21st on K1 vs K2 were 1.324±1.079, 7.607±8.922, and 5.220±1.653 vs 4.705±3.369, 4.184±5.512, and 1.797±0.898 ng/mL (P>0,05), respectively
Seminar Nasional Peternakan dan Veteriner 1998: 268-269
Summary
Pengamatan birahi dilakukan tiga kali sehari pada pukul 06.00, 12.00, dan 18.00 WIB secara visual dibantu dengan pejantan selama empat hari sejak penyuntikan PGF2α terakhir. Sampel darah sebanyak 5 ml diambil dari vena jugularis menggunakan disposible syringe 5 ml, kemudian dimasukkan dalam microtube dan ditempatkan dalam termos es. Sampel darah dibawa ke Laboratorium Riset Fakultas Kedokteran Hewan Universitas Syiah Kuala untuk diambil serumnya dengan cara sentrifugasi dengan kecepatan 3.000 rpm selama 15 menit. Serum diambil dari tabung dengan pipet mikro dan dimasukkan dalam microtube, disimpan dalam freezer -20° C sampai dilakukan pengukuran progesteron. Pengukuran konsentrasi progesteron Tiap sumur microplate ELISA dimasukkan 25 μl larutan standar, sampel, dan kontrol, kemudian ditambahkan 200 μl reagen konjugat progesteron pada tiap sumur, selanjutnya diinkubasi selama 60 menit pada suhu ruang. Tiap sumur dimasukkan sebanyak 100 μl larutan substrat solution dan diinkubasi selama 15 menit pada suhu ruang. Nilai absorbansi dibaca pada ELISA reader dalam waktu 10 menit setelah ditambahkan stop solution dengan absorbansi 450±10 nm
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