Abstract

The N-terminal region of bovine serum albumin (Asp-Thr-His-Lys) is known to provide a specific binding site for Cu(II) ions, with the histidine residue thought to be mainly responsible for the specificity. Thiomolybdates have been found to increase the binding affinity of Cu(II) to some serum albumins. As part of a series of studies to study the interactions between Cu(II), thiomolybdates and bovine serum albumin, we have performed the syntheses and characterization of small model peptides such as His-Lys, Thr(Ac)-His-Lys and Thr-His-Lys. Proton NMR spectra have been monitored in H(2)O solution as a function of pH and added Cu(II) concentration. Reliable K(a) values for His-Lys and Thr(Ac)-His-Lys have been established. Probable binding sites of Cu(II) and the relative strengths of binding to these peptides are also discussed.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call