Peptides from myelin basic protein as substrates for adenosine 3′, 5′-cyclic monophosphate-dependent protein kinases

Abstract

A simple electrophoretic assay demonstrated that peptides from enzymic digests of the basic protein of human myelin were effective substrates for adenosine 3′, 5′-cyclic monophosphate-dependent protein kinases from bovine cardiac muscle and brain. From a peptic digest a peptide of 17 amino acid residues was isolated and when used as a substrate a K m of 1.9 × 10 −4M was found for the cardiac kinase.