Abstract
A label-free biosensor based on surface plasmon resonance (SPR) has been developed to evaluate the potential of peptide ligands as biorecognition molecules for the detection of bacterial toxins. A 24-mer peptide, previously identified from a phage display library and modified for better binding, was used for the detection of staphylococcal enterotoxin B (SEB). The peptide was immobilized covalently onto the gold-coated surface modified with self-assembly monolayer. The limit of detection (LOD) for SEB was found to be 20 μg/mL. The selectivity of the peptide-based sensor for SEB was verified by injecting bovine serum albumin (BSA), and the sensor response for the BSA was negligible. Further, to probe the effectiveness of the sensor in complex food environments, it was tested with milk samples spiked with SEB, showing that it is applicable to complex food matrices. The results of this study demonstrate the potential of peptides, selected from phage display libraries, as recognition agents for detecting bacterial toxins using biosensors.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
