Abstract
The aim of the present study was to identify a high affinity InsP 4 receptor in membranes from cerebellum or brain from several species. In samples obtained from sheep, beef, human and rat, a 42 kDa protein represents an Ins(1,3,4,5)P 4 receptor, similar to the InsP 4 receptor from pig cerebellum, which we have described previously using an InsP 4-photoaffinity analogue (Reiser et al., Biochem. J. 1991, 280, 533). CNBr cleavage of the pig receptor protein for peptide sequencing revealed peptide sequences which demonstrate that the 42 kDa InsP 4 receptor is a novel protein. Two synthetic peptides derived from the pig receptor were used to generate peptide-specific antisera which recognized also the intact receptor protein from pig. The two antisera showed different reactivity with the InsP 4 receptor purified from pig and human compared to sheep, beef and rat. This indicates a species heterogeneity of this protein.
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