Abstract
This study aimed to understand the role of the mucus layer (a biological hydrogel) in the transport mechanisms of peptides. Using established in vitro models, the mucin-binding activity and mucus-permeating property of peptides were determined. Uncharged peptides with relatively high hydrophilicity, including MANT, TNGQ, and PASL, as well as cationic peptides, including KIPAVF and KMPV, possessed strong mucin-binding activity. Contrarily, uncharged peptides with high hydrophobicity index, including YMSV and QIGLF, exhibited weak mucin-binding activity. Only TNGQ, which has high Boman index and hydrophilicity, showed a high biosimilar mucus-permeating property with a permeability of 96 ± 30% after 60 min. TNGQ showed the potential for high bioavailability due to the high mucin-binding and biosimilar mucus-permeating activities.
Highlights
Bioactive peptides, usually consisting of 2–20 amino acid residues, are commonly released from the parent proteins by enzymatic hydrolysis or microbial fermentation [1].In vitro and in vivo studies have demonstrated that bioactive peptides exert health-promoting properties, including antihypertensive, antioxidant, anti-inflammatory, immunomodulatory, antidiabetic, and anticancer activities [1,2]
The Caco-2 cell line lacks the mucus layer that is located on the surface of the epithelial membrane and functions as a protective barrier [6]
TNGQ was more than 100%, possibly due to the impurity of BSA in the biosimilar mucus rather than targetedremoved peptides by the OPA as method
Summary
Usually consisting of 2–20 amino acid residues, are commonly released from the parent proteins by enzymatic hydrolysis or microbial fermentation [1]. Still not known viatight tight junctions, transcytosis, andtranscellular passive transcellular It ishow stillpeptides not known ho interact with mucin penetrate the mucusthe layers before transporting across the underlying tides interact withand mucin and penetrate mucus layers before transporting across the und epithelium. Mucins are the major functiona the transport of bioactive peptides [11] To date, it is still not well understood how peptides interact mucin the mucus layers before transporting across ponents of with mucus thatand arepenetrate responsible for the viscoelastic properties of the the mucus underlying epithelium, as illustrated in Figure [5]. Boman index indicated the binding potential co-culture monolayers) has been used as a more physiologically relevant model to ate the transport of bioactive peptides [11] To date, it is still not well understood peptides interact with mucin and penetrate the mucus layers before transporting the underlying epithelium, as illustrated in Figure 1 [5].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
