Peptide-modified carbon dot aggregates for ultrasensitive detection of lipopolysaccharide through aggregation-induced emission enhancement

Abstract

This study fabricated yellow-emitting CDs (Y-CDs) by hydrothermal treatment of citric acid and urea and applied them as a fluorescence turn-on platform for sensitive and selective detection of lipopolysaccharide (LPS) based on the non-shifted AIEE of peptide-stabilized CD aggregates. The designed peptide (named K3) consisting of aggregation-active and LPS-recognition units triggered the aggregation of Y-CDs, switching on their fluorescence through the blue-shifted AIEE process. The formed K3-stabilized Y-CD aggregates (K3-YCDAs) specifically interacted with LPS at neutral pH, demonstrating that the sequence of the decorated peptide was highly connected with their selectivity and sensitivity. The K3-YCDAs provided a fast response time (within 5 min) to detect LPS with a quantification range of 0.5–100.0 nM and a limit of detection (LOD, signal-to-noise ratio of 3) of 300.0 pM. By integrating ultrafiltration membranes as a concentration device with K3-YCDAs as a sensing probe, the LOD for LPS was further reduced to 3.0 pM. The determination of picomolar levels of plasma LPS by the K3-YCDAs coupled to the centrifugation ultrafiltration was demonstrated to fall within the specificity range of clinical interest for sepsis patients. Also, the K3-YCDAs served as a fluorescent probe to selectively image and quantify E. coli cells. The distinct advantages of the K3-YCDAs for LPS include fast response time, wide linear range, low detection limit, and excellent selectivity compared to previously reported sensors.