Abstract
Background and objectives: Nearly 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized. Materials and Methods: To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice. Results: Peptide PF3 and PF4 displayed high docking G-scores (−9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response. Conclusions: Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.
Highlights
20–30% of the world’s population is suffering with allergic diseases viz., allergic rhinitis, atopic dermatitis, and bronchial asthma [1]
Protein Data Bank (PDB) structure 5FDS: the top hit from the blast analysis was selected as a template and energy based model was developed (Figure 1a)
The structural alignment of the model was evaluated through Ramachandran plot and ERRAT score
Summary
20–30% of the world’s population is suffering with allergic diseases viz., allergic rhinitis, atopic dermatitis, and bronchial asthma [1]. There are many different triggers of allergy and pollen grains of tree, grass, and weed plants are one of the major causative agents for respiratory allergic diseases, in particular asthma and rhinitis [2]. More than 50% of the patients were prone to major allergens, while minor or panallergens sensitization was ~5–40% [3]. An allergic protein of 12–15 kDa, was identified in tree, grass, weed pollens, latex, and plant-derived foods [4]. 20–30% of the world’s population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized.
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