Abstract
BackgroundInfectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. Vaccination plays a vital role in controlling the disease. The efficacy of vaccination in chicken flocks can be evaluated by detecting neutralizing antibodies with the neutralization test. However there are no simple and rapid methods for detecting the neutralizing antibodies.ResultsIn this study, a peptide enzyme-linked immunosorbent assay (pELISA) as a possible alternative to the neutralization test for evaluating the immune response to IBV vaccine was developed. The pELISA could indirect evaluate neutralizing antibody titers against different types of IBV in all tested sera. The titers measured with the pELISA had a coefficient of 0.83 for neutralizing antibody titers.ConclusionsThe pELISA could detect antibodies against different types of IBV in all tested sera. The pELISA has the potential to evaluate samples for IBV-specific neutralizing antibodies and surveillance the infection of IBV.
Highlights
Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection
The results showed that immune sera against other viruses, such as NDV, avian leukosis virus (ALV), MDV, avian influenza virus (AIV), IBDV, gosling plague virus (GPV), reticuloendotheliosis virus (REV), ILTV and EDS-76V, were negative in the peptide enzyme-linked immunosorbent assay (pELISA) (Fig. 1)
The inter-assay and the intra-assay coefficient of variation of pELISA were less than 10 %
Summary
Infectious bronchitis virus (IBV), a coronavirus, is one of the most important poultry pathogens worldwide due to its multiple serotypes and poor cross-protection. The correlation of antibody titers between indirect ELISA and neutralization tests has been studied in Zika virus and human papillomavirus [13, 14]. ELISA for detecting antibodies against IBV have been developed with the whole virion or recombinant S1 proteins, N proteins and nonstructural proteins [15,16,17]. These ELISA methods have achieved good results in detecting IBV antibody. These methods could not evaluate the neutrolization antibody level in immunized chickens. Serological alternatives to neutralization tests for IBV have not been studied
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