Abstract
Graphene oxide (GO) is extensively studied as a template material for mesenchymal stem cell application due to its two-dimensional nature and unique functionalization chemistries. Herein, a new type of peptide-conjugated multilayer graphene oxide (peptide/m-GO film) was fabricated and used as biomaterial for culturing human Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs). The characterization of the peptide/m-GO films was performed, and the biocompatibility of the WJ-MSCs on the peptide/m-GO films was investigated. The results demonstrated that the peptide conjugate on the m-GO film did not hamper the normal growth of WJ-MSCs but supported the growth of WJ-MSCs after the 6-day culture period. In addition, the osteogenic differentiation of WJ-MSCs on the peptide/m-GO films was enhanced as compared with the parent m-GO film. Therefore, such peptide-conjugated m-GO films could provide a highly biocompatible and multifunctional 2D material to tailor the potential application of WJ-MSCs in bone tissue regeneration.
Highlights
Mesenchymal stem cells (MSCs) have been studied extensively in regenerative medicine.MSCs can be either applied alone or integrated with scaffolds in the treatment of several diseases, including bone and cartilage dehiscence, wound therapy, and cardiovascular and neural disorders [1,2,3,4]
The use of human Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) isolated from human umbilical cord tissue, which is considered a medical waste discarded at birth, is noninvasive and involves no ethical concerns
We investigated the potential of our modified m-graphene oxide (GO) film in biomedical applications
Summary
Mesenchymal stem cells (MSCs) have been studied extensively in regenerative medicine.MSCs can be either applied alone or integrated with scaffolds in the treatment of several diseases, including bone and cartilage dehiscence, wound therapy, and cardiovascular and neural disorders [1,2,3,4]. Mesenchymal stem cells (MSCs) have been studied extensively in regenerative medicine. MSCs can be isolated from various sources, including bone marrow, adipose tissues, mobilized peripheral blood, skin epithelium, and umbilical cord [5]. WJ-MSCs have been the subject of intensive research as a potential cell source for mesenchymal stem-cell-based therapy in the fields of tissue engineering and regenerative medicine [9,10]. In previous studies, WJ-MSCs were reported to have a lower osteogenic differentiation potential as compared with bone marrow-isolated mesenchymal stem cells (BM-MSCs) [11,12]. Graphene oxide (GO) has been extensively studied for its adhesive ability and biocompatibility with MSCs to regulate the cell signaling that triggers cell responses, including cell proliferation and differentiation [14,16,17,18]. The GO surface possesses greater interactions with biomacromolecules via covalent or noncovalent interactions, such as π–π interactions, van der Waals forces, ionic interactions, and hydrogen bonding [21,22,23]
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