Abstract
THE PROTEIN FACTOR S, PREVIOUSLY SHOWN TO STIMULATE POLYPEPTIDE CHAIN TERMINATION IN BACTERIAL EXTRACTS, HAS TWO EFFECTS UPON THE COMPLEX FORMED BETWEEN RIBOSOMES, RELEASE FACTOR, AND TERMINATOR (TRINUCLEOTIDE) CODON: (1) in the absence of GTP or GDP, S stimulates formation of an [R.UAA.ribosome] intermediate, and (2) in the presence of GTP or GDP, S participates in dissociation of this intermediate. Factor S can stimulate fMet release from [fMet-tRNA(f).AUG.ribosome] intermediates in either the presence or absence of GTP or GDP. A model is proposed which relates the in vitro effects of S +/- GTP (or GDP) on fMet release to the effects of S +/- GTP (or GDP) on the binding and dissociation of R factor from ribosomes.
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