Peptide-Capped Gold Nanoparticle for Colorimetric Immunoassay of Conjugated Abscisic Acid

Abstract

The pentapeptide Cys-Ala-Leu-Asn-Asn (CALNN) has been proved to be a powerful tool to stabilize the AuNPs. These CALNN-capped AuNPs have been used to develop various bioanalysis platforms. In this paper, the CALNN-capped AuNPs are proved to be a robust tool for aggregation-based colorimetric immunoassays as well. A colorimetric immunoassay strategy based upon the antibody-induced assembly of functionalized AuNPs for Abscisic Acid glucose ester (ABA-GE) determination has been developed. The ABA-functionalized AuNPs aggregate in the presence of specific antibody, accompanied by a color change of the solution. The color change is competitively inhibited by ABA-GE. The interparticle distance in aggregates is small due to the thin peptide layer on the AuNPs surface, and it is determined by the "Y" shape antibody linker as well. As a result of that, an obvious color change in the immunoassays is observed. Under the optimized conditions, a linear response range from 5 nM to 10 μM for ABA-GE determination is obtained, and the limit of detection (LOD) is evaluated to be 2.2 nM. This method is simple, homogeneous, and has potential for visual detection of ABA-GE.