Abstract
In order to clarify one of the biological functions of pork, we investigated whether a peptic hydrolysate of denatured porcine crude myosin showed inhibitory activity against angiotensin I-converting enzyme (ACE), which contributed to hypertension. Our results indicated that this hydrolysate showed relatively strong activity, and we therefore attempted to separate the involved peptides, which were considered to be active substances. To isolate these active peptides, the hydrolysate was separated using a solid- phase separation, gel filtration high-performance liquid chromatography (HPLC), and two kinds of reverse phase HPLC. In each stage of separation, many fractions were detected, almost all of which showed ACE inhibitory activity. Thus, we suggested that the activity of the hydrolysate as a whole was a result of the activities of the many individual peptides. Six peaks were distinguished, with yields from 34 to 596 ppm of original crude myosin. In addition to the six peaks, many other active fractions were found throughout the separation steps, strongly suggesting that whole porcine crude myosin itself had ACE inhibitory activity. Moreover, pork as food was considered to function as an ACE inhibitory material in vivo, because pork proteins consist primarily of crude myosin, which included almost all the myofibrillar structural proteins. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 9 : 1384-1389)
Highlights
The tertiary function of food, which contributes to the modulation of our physiological systems, has been widely investigated, and certain functional foods have already been developed in Japan (Arai, 1996)
Our results indicated that this hydrolysate showed relatively strong activity, and we attempted to separate the involved peptides, which were considered to be active substances
angiotensin I-converting enzyme (ACE) plays an important role in the regulation of blood pressure; it contributes to hypertension by converting angiotensin I to angiotensin II
Summary
The tertiary (physiologically active) function of food, which contributes to the modulation of our physiological systems, has been widely investigated, and certain functional foods have already been developed in Japan (Arai, 1996). In each stage of separation, many fractions were detected, almost all of which showed ACE inhibitory activity. In addition to the six peaks, many other active fractions were found throughout the separation steps, strongly suggesting that whole porcine crude myosin itself had ACE inhibitory activity.
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