Pepsin measurement in saliva of the healthy adults for detection of laryngopharygeal reflux

Abstract

Objective To measure the pepsin level in saliva of the healthy adults in order to find a method to diagnose the laryngopharyngeal reflux disease by measurement of the pepsin level in the saliva of patients. Methods From Sept.1 2016 to Sept.30, 2016 in Department of Otolaryngology Head Neck Surgery, Navy General Hospital, 50 healthy adults were randomly recruited as subjects, and the saliva was collected into 1.5ml sterile EP tubes after awaking in the morning, and the tubes were placed in a refrigerator of -28 ℃ within half an hour, meanwhile the longest freezing time was less than 2 weeks.The level of pepsin in saliva was measured by human pepsin ELISA kit. Results Among the 50 subjects, the average content of pepsin in saliva was(24.22±6.94)ng/ml and the 95% confidence interval was(22.18, 26.06)ng/ml.The average content of pepsin in saliva was(22.78±1.35)ng / ml and 95% confidence interval was 20.00, 25.57)ng/ml in male group.The average content of pepsin in saliva was(25.67±1.39)ng/ml and 95% confidence interval was(22.80, 28.54)ng/ml in female group. Conclusion The pepsin can be detected in the saliva of the healthy adults, the level of the pepsin in the saliva of the patients may be used to the diagnosis of the laryngopharyngeal reflux disease in the future. Key words: Stomach; Pepsin; Laryngopharyngeal; Reference value