Abstract
The initial stop of d-xylose metabolism by cell-free extracts of Oospora lactis (Geotrichum candidum L.) involves the reduction of xylose to xylitol by TPNH, followed by the DPN-linked oxidation of xylitol to xylulose. Xylulose is phosphorylated by added ATP, thus entering the respiratory pentose phosphate cycle. Also, d-ribose is reduced by the cell-free extracts in the presence of TPNH. The enzymically formed product, d-ribitol, has been isolated and identified. Little, if any, ribitol DPN-linked oxidation was instead observed. These data are discussed from the point of the utilization of d-ribose by the resting cells of Oospora lactis without any increased oxygen uptake above the endogenous rate.
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