Penicillium subrubescens adapts its enzyme production to the composition of plant biomass

Adiphol Dilokpimol,Mao Peng,Marcos Di Falco,Thomas Chin A Woeng,Rosa M.W Hegi,Zoraide Granchi,Adrian Tsang,Kristiina S Hildén,Miia R Mäkelä,Ronald P De Vries

doi:10.1016/j.biortech.2020.123477

Adiphol Dilokpimol, Mao Peng + Show 8 more

Open Access

https://doi.org/10.1016/j.biortech.2020.123477

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Abstract

Penicillium subrubescens is able to degrade a broad range of plant biomass and it has an expanded set of Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison to other Penicillium species. Here we used exoproteome and transcriptome analysis to demonstrate the versatile plant biomass degradation mechanism by P. subrubescens during growth on wheat bran and sugar beet pulp. On wheat bran P. subrubescens degraded xylan main chain and side residues from Day 2 of cultivation, whereas it started to degrade side chains of pectin in sugar beet pulp prior to attacking the main chain on Day 3. In addition, on Day 3 the cellulolytic enzymes were highly increased. Our results confirm that P. subrubescens adapts its enzyme production to the available plant biomass and is a promising new fungal cell factory for the production of CAZymes.

Highlights

Plant biomass is one of the most abundant, valuable and sustainable polymeric substrates, and is currently the most suitable resource for renewable bioenergy and biochemical production
Understanding how Carbohydrate Active enzyme (CAZyme) are produced by P. subrubescens cultures during growth on different plant biomass is critical to develop this fungus as a novel fungal cell factory
To gain insight into how P. subrubescens degrade plant biomass, we report a comparative exoproteome and transcriptome of P. subrubescens cultures grown on wheat bran and sugar beet pulp in this study

Summary

Introduction

Plant biomass is one of the most abundant, valuable and sustainable polymeric substrates, and is currently the most suitable resource for renewable bioenergy and biochemical production. Genomic analysis of P. subrubescens revealed an expanded set of Carbohydrate Active enzyme (CAZyme)-encoding genes in comparison with the related Penicillium species, but more similar in general numbers to A. niger and other aspergilli (Peng et al, 2017) This expansion does not occur across all CAZy families, but is rather specific to hemicellulose, pectin and inulin degrading enzymes, occurring in glycoside hydrolase (GH) family GH1, GH11, GH12, GH29, GH32, GH36, GH43, GH51, GH54, GH62 and GH67, as well as carbohydrate esterase (CE) family CE8 (Peng et al, 2017). We reported the extracellular enzyme activity and saccharification abilities of P. subrubescens cultures grown on wheat bran and sugar beet pulp (Mäkelä et al, 2016) These two feedstocks differ in their carbohydrate composition. To gain insight into how P. subrubescens degrade plant biomass, we report a comparative exoproteome and transcriptome of P. subrubescens cultures grown on wheat bran and sugar beet pulp in this study

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