Abstract
Penicillium sclerotigenum Yamamoto1 (CMI, 68,616) has been shown to produce griseofulvin. The fungus causes tuber rots of the Chinese yam, Dioscorea batatas. It was grown in Roux bottles in a medium containing 75 g ‘Dextrosol’, 2.9 g potassium nitrate, 5.0 g potassium dihydro-orthophosphate, 1.0 g magnesium sulphate heptahydrate, 1.0 g sodium chloride, 0.5 g ‘Difco’ yeast extract, and 1 ml. of minor element concentrate (containing 0.1 g ferrous sulphate heptahydrate, 0.015 g copper sulphate pentahydrate, 0.05 g zinc sulphate heptahydrate, 0.01 g manganese sulphate heptahydrate and 0.01 g. sodium molybdenate, in 100 ml.) made up to 1 l. with distilled water. The mycelium and broth were extracted with chloroform after 26 days growth.
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