Abstract
A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have