Penicillin Binding Proteins3 and 4 Relations between Resistance Phenotypes and mecA, TEM genes expression in Staphylococcal aureus

Abstract

This study was to determine the relationship between pbp3 and pbp4 gene compared with mecA and TEM resistance genes expression patterns. Total 134 clinical S. aureus strains were subjected to 19 antimicrobial susceptibility tests. We detected resistance to methicillin (mecA), penicillin (blaTEM) and expression of pbp (Penicillin-binding proteins) genes. We were compared blaTEM, extended spectrum, carbapenem related genes and types of SCCmec identified. Total of 134 clinical S. aureus strains, 79 (58.96%) in methicillin resistance, and 77 strains carried mecA. Prevalence rates of blaTEM and pbp genes were 107/134 (79.85%) and 128/134 (95.52%). Multiplex PCR results revealed that the predominant SCCmec type among 77 mecA-positive MRSA strains were similer too SCCmec type II 41.56% (32/77) and type IVA 40.26% (31/77). Prevalence rates of type IVb, IVd and non-typable were 18.18% (14/77), respectively. From a total of 77/134 (57.46%) MRSA isolate strains, 35/77 (45.46%) were positive for extended spectrum, 40/77 (51.95%) for cephalosporins, and 35/77 (45.46%) for carbapenems. The predominant SCCmec type II had more carbapenem resistances than IVA, IVb and IVd. TEM and mecA gene expression were not correlated with pbp gene, and the properties of drug resistance were appeared not associated with pbp3, 4 genes.