Penggunaan Imunostik sebagai Uji Serologi untuk Deteksi Brucella abortus pada Sapi (APPLICATION IMMUNOSTICK ASSAY FOR SEROLOGICAL TEST BRUCELLA ABORTUS IN BOVINE)

Dhevie Kenny Astarina,Eko Sugeng Pribadi,Fachriyan Hasmi Pasaribu

doi:10.19087/jveteriner.2018.19.2.169

Dhevie Kenny Astarina, Eko Sugeng Pribadi + Show 1 more

Open Access

https://doi.org/10.19087/jveteriner.2018.19.2.169

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Abstract

Serological test is one of diagnostic method to detect pathogenicity brucella. Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA). Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to know sensitivity and specificity of immunostick that were used to detecte antibody Brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared with ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with sensitivity 100%. Immunostick specifity were 45,45% for B. abortus S99(1) antigen; 78,79% for B. abortus S99(2) antigen and 51,52% for B. abortus S19 antigen. When the test compared with ELISA, the sensitivity 82,86% and the spesifity were 52,31% for B. abortus S99(1) antigen; 93,54% and 79,71 for B. abortus S99(2) antigen and 82,86% and 58,46% for B. abortus S19 antigen.

Highlights

PENDAHULUAN Metode baku untuk mendiagnosis brucellosis adalah mengisolasi dan mengidentifikasi bakteri penyebab
Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA)
Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection

Summary

Jurnal Veteriner

PENDAHULUAN Metode baku (gold standard) untuk mendiagnosis brucellosis adalah mengisolasi dan mengidentifikasi bakteri penyebab. Oleh karena itu diagnosis laboratorium yang dilakukan pada umumnya menggunakan metode pengujian serologis atau molekuler dari serum atau cairan tubuh. Imunostik sebagai uji serologik yang memiliki tingkat kemudahan dan akurasi yang setara dengan ELISA dapat digunakan untuk uji serologis cepat guna mendeteksi brucellosis di lapangan. Perbandingan uji dilakukan dengan membandingkan hasil ELISA dan imunostik menggunakan 100 sampel terdiri dari 25 seropositif, 60 seronegatif dan 15 unknown sera. Deteksi antibodi terhadap sampel sapi lapang dilakukan dengan beberapa uji yaitu RBT, CFT, ELISA, dan Imunostik. Imunostik yang telah siap pakai direndam ke dalam tabung dan diinkubasi pada suhu ± 27oC selama 10 menit. Imunostik direndam dalam tabung yang berisi konjugat antibovine IgG-HRP (1:3000) (Sigma®, USA) dan diinkubasi pada suhu ±27oC selama 10 menit. Adapun akurasi pengujian dievaluasi dengan The Curve Diagnostic Accurasy (AUC) (Simundic, 2009; Dohoo et al, 2010)

HASIL DAN PEMBAHASAN Penentuan Kelompok Seropositif dan Seronegatif

Teknik uji

Komponen g kurang

Pengenceran serum