Abstract
Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants. To be more effectively applicable in the field, some experiments have been employed to formulate T. pseudokoningii in a biofungicide formulation amended with organic matter as its main nutrient resource, zealot as a carrier agent and cocoyam powder as a mixture agent. A research has been conducted to study the effect of various organic matters and their combinations in a biofungicide formulation of T. pseudokoningii on growth inhibition to G. boninense in-vitro and to obtain the best organic matters and their combinations in enhancing the growth of T. pseudokoningii and yet inhibiting G. boninense. The research has been conducted in the Laboratory of Plant Pathology, Agriculture Faculty, University of Riau from May to August 2012, using a completely randomized design consisting of 15 treatments (bagasse, rice husk, shrimp shell, dregs, and their combinations) and 3 replications. The data were statistically analyzed using analysis of variance, followed by Duncan’s New Multiple Range Test (DNMRT). The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro. Rice husk, bagases, bagasse+rice husks, and bagasse+dregs were the best organic matters in enhancing the growth of T. pseudokoningii and its capacity to inhibit G. boninense in-vitro. It can also be concluded that shrimp shell, bagasse+shrimp shell, rice husk+shrimp shell, shrimp shell+dregs and rice husk+shrimp shell+dregs totally inhibited the growth of T. pseudokoningii in the biofungicide formulation.
Highlights
Trichoderma pseudokoningii has been applied as a biocontrol agent against fungal plant pathogen, such as Ganoderma boninense, the cause of stem rot disease on palm oil plants
The results indicated that organic matters and their combinations in the biofungicide formulation significantly affected the antagonistic capacity of T. pseudokoningii in inhibiting the growth of G. boninense in vitro
Pengaruh pemberian Trichoderma viride dan sekam padi terhadap penyakit rebah kecambah di persemaian cabai
Summary
Penelitian ini dilaksanakan di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Riau, Simpang Baru Panam Pekanbaru. Sedangkan jumlah tepung dari ampas tebu, limbah udang, sekam padi dan dregs untuk masing-masingnya yang digunakan adalah sebanyak 1,125 g, 100 g dari masingmasing bahan organik dan 50 g zeolit dimasukkan ke dalam kantong plastik polyethylen. Pengamatan dilakukan setiap hari terhadap koloni jamur T. pseudokoningii yang terdapat dalam formulasi biofungida yang ditumbuh pada cawan petri untuk tiap unit percobaan. Pengukuran diameter koloni dilakukan ketika koloni jamur yang tumbuh pada medium PDA yang telah diinokulasikan dengan formulasi biofungisida sesuai perlakuan telah memenuhi cawan petri. Kemampuan penghambatan terhadap pertumbuhan jamur G. boninense pada masing-masing formulasi biofungisida dihitung sampai ada jamur T. pseudokoningii pada setiap perlakuan yang telah tumbuh hingga ke bagian pinggir koloni jamur G. boninense setelah ditumbuhkan pada medium PDA. Pengukuran pH pada formulasi biofungisida dilakukan dengan mengambil sampel formulasi biofungisida (sesuai dengan perlakuan) sebanyak 5 g yang dilarutkan ke dalam 10 mL akuades dan diukur menggunakan pH meter
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