Pengaruh VCO terhadap hitung jenis leukosit, kadar glukosa dan kreatinin darah Mus musculus Balb/c hiperglikemi dan tersensitisasi ovalbumin

Abstract

Handajani NS, Dharmawan R. 2009. Effect of VCO to leucocyte differential count, glucose levels and blood creatinine of hyperglycemic and ovalbumin sensitized Mus musculus Balb/c. Nusantara Bioscience 1: 1-10. Chemical medicines and insulin can decrease glucose blood level on hyperglycemic patients with macro vascular side effect. Diabetes and allergy incidences are influenced by quality and quantity of leucocytes. Lauric acid within VCO reports decreased glucose blood level of diabetes and some allergy incidents. The purpose of the study is to know the effect of VCO on glucose blood level, differential leucocytes count and creatinine blood level on hyperglycemic and normoglicemic ovalbumin sensitized mice. Forty five (45) male (mice) of Mus musculus Balb/c with average weight of 35 g are divided into 9 groups with 5 repetitions, those are 4 non alloxan groups and 5 alloxan induced hyperglycemic groups. On 22nd day to 36th day they are sensitize to ovalbumin as allergen. Blood sample was obtained by orbital vena using heparin as anti coagulant in order measuring glucose blood level by GOD method to 6 times, on 1st, 4th, 18th, 22nd, 32nd and 37th days, then are tested by ANOVA followed by DMRT 0.05. On 37th day, differential leucocytes are determined, blood level are counted, and then compared to normal value. The result of this study were that within differential leucocytes count of hyperglycemic mice, neutrophile percentage were much lower than the normal value (3.22%), and lymphocyte percentage were much higher than the normal value (94.54%). Consumed 0.003 mL/35 g VCO more 18 days decreased glucose blood level on hyperglycemic mice, decreased basophile percentage of ovalbumin sensitized mice, normalized neutrophile percentage no increased creatinine blood level.