Abstract
The aim of this study was to determine the effect of superovulation induction through Pregnant Mare’s Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG) injection to increased birth number in Campbell’s hamsters (Phodopus campbelli). This study used 10 unpregnant female Campbell’s hamster, aged 4-6 months, weighing of 25-30 grams. Hamsters were divided into K1 and K2, each consist of five hamsters. K1 injected with 1 ml physiologic NaCl, while K2 intraperitoneally injected with PMSG and hCG in 5 IU. Mating was held after injection. Male and female hamsters were put in one box with ration of 1:1. The observation of vagina plug was done in order to confirm whether the mating successes or not. The average amount of fetus birth through superovulation on K1 and K2 were 3.40±1.3 and 7.80±1.1, respectively. In conclusion, the superovulation induction using the combination of PMSG and hCG increase birth number on Campbell’s hamsters.
Highlights
The aim of this study was to determine the effect of superovulation induction through Pregnant Mare's Serum Gonadotropin
Hamsters were divided into K1
K1 injected with 1 ml physiologic NaCl
Summary
Hewan coba yang digunakan diperoleh dari pet shop yang berada di Medan Sumatera Utara. Hewan coba yang digunakan adalah 10 ekor hamster Campbell betina dengan bobot badan 25-30 g, umur 4 bulan, dan tidak bunting. Seluruh hamster dibagi dalam dua kelompok yaitu kelompok kontrol (K1) dan kelompok perlakuan (K2), masing-masing berjumlah lima ekor hamster. Superovulasi dan Perkawinan Kelompok K1 diinjeksi dengan NaCl fisiologis sebanyak 1 ml, sedangkan K2 diinjeksi dengan 5 IU PMSG (FolligonTM, Intervet, Boxmeer, Holland) dan 5 IU hCG (ChorulonTM, Intervet, Boxmeer, Holland). Hamster pada K1 dan K2 disatukan dengan hamster jantan. Pola perlakuan perkawinan sesuai petunjuk Rosadi et al (2008). Jantan dan betina dicampurkan dengan dengan perbandingan 1:1. Untuk memastikan keberhasilan perkawinan dilakukan pengamatan vagina plug
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