Abstract
Free radical from cigarrete smoke can cause lipid peroxidation that could lead to tissue damage. Tissue damage can be identified by increased levels of MDA (Malondialdehyde). Leavesof buas-buas (Premna cordifolia. Linn ) has an antioxidant activity in invitro study. This study aimed to analyze the effect of P. cordifolia leaves extract on the levels of malondialdehyde (MDA) after exposure to cigarette smoke. The simplicia extracted by maseration using 70% ethanol. Twenty four wistar strain of Rattus novergicus were divided into six groups , i.e. : normal group without any treatment, positive control group with cigarette smoke exposure and vitamin e treatment, negative control groupwith only cigarette smoke exposure, treatment groupwith cigarette smoke exposure and ethanolic extract from P. cordifolialeaves with doses 200; 400; 600 mg/kg of body weight/day. All rats were treated for 14 days using 3 cigarette non filetered each day. After 14 days, plasma MDA was measured in this research. Measurement of plasma MDA are using Spectrophotometer UV-Vis in 532.2 nm. MDA level in this study was analyzed by the Kruskal-Wallis and Mann-Whitney test. The result showed that plasma MDA level of groupreceived ethanol extract of P. cordifolia leaves doses at 600 mg/kg of body weight/day significantly (p<0.05)lower than negative control group and not significantly differ (p<0.05) to normal group and positive control group.
Highlights
cause lipid peroxidation that could lead to tissue damage
Tissue damage can be identified by increased levels of MDA
This study aimed to analyze the effect of P. cordifolia leaves extract on the levels
Summary
Latar Belakang Merokok merupakan salah satu gaya hidup utama yang berpengaruh pada kesehatan manusia. Asap rokok mengakibatkan stress oksidatif yang ditandai dengan meningkatnya radikal oksidan dan reaksi inflamasi berupa peningkatan jumlah total leukosit dan neutrofil darah perifer. Radikal bebas dari asap rokok menyebabkan peroksidasi dari asam lemak ganda tak jenuh membran sel yang memperkuat stres oksidatif selama merokok. Penggunaan rokok dalam waktu yang lama dapat menyebabkan ketidakseimbangan oksidanantioksidan sistemik yang ditandai dengan adanya hasil dari peroksidasi lipid yaitu MDA (Malondialdehid). Antioksidan merupakan senyawa yang berguna mengatasi kerusakan oksidatif akibat radikal bebas dalam tubuh.Daun buas-buas merupakan salah satu alternatif bahan makanan sumber antioksidan yang belum banyak diteliti kegunaannya.Masyarakat umumnya menggunakan daun buas-buas (Premna cordifolia) sebagai bumbu masakan dan makanan.Buas-buas memiliki kandungan kimia fenolik dan flavonoid yang berperan dalam aktivitas antioksidan.Ekstrak methanol daun buas-buas memiliki aktivitas antioksidan yang sangat aktif dengan nilai IC50 sebesar 31,91± 0,43 μg/ml [8, 9, 10]. Jurnal Fitofarmaka Indonesia, Vol 1 No 2 jantan yang diberi paparan asap rokok dan ekstrak etanol daun buas-buas
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