Abstract
Effect of thawing technique to quality frozen semen spermatozoa in bali, madura and PO cattleABSTRACT. The experiment was conducted from November 2011 to January 2012 in BBIB Singosari. The study aims was to determine the effect of thawing techniques on the quality frozen bovine sperm Bali, Madura and PO cattle. This material used 90 frozen straw of three cattle from the three breed. The method is an experiment with three treatments and 10 replications for frozen to thawing in temperature water is 5 0C of duration 60 seconds, 15 0C duration 30 seconds and 37 0C duration 15 seconds. Variables observed in this study were sperm quality are motility, viability and abnormality. Data in percentage transformed in to arcus sinus were analyzed using Completely Randomized Block Design (CRBD) with replication as blocks of 10x. The results showed the thawing at 37 0C duration 15 seconds to highly significant effect (P0,01) viability and motility and significant effect (P0.05). The abnormalities, no significant effect (P0,05). Breed cattle not significant effect to quality. It can be concluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle. Thawing using 37 0C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm.
Highlights
Randomized Block Design (CRBD) with replication as blocks of 10x.The results showedthe thawing at 37 0C duration 15 seconds to highly significant effect (P
Breed cattle not significant effect to quality.It can be cocluded that thawing techniques impact the motility and viability in frozen bovine semen spermatozoa Bali, Madura and PO cattle
Thawing using 370C temperature water with duration of 15 seconds is the best method to obtain the quality of spermatozoa post thawing frozen sperm
Summary
Tempat dan Waktu Penelitian Penelitian ini telah dilaksanakan di Laboratorium BBIB Singosari Malang, selama 1 Bulan (November 2011 – Desember 2011). Materi dan Metode Penelitian ini menggunakan 90 straw semen beku dari sapi Bali, sapi Madura dan sapi PO yang diperoleh dari BBIB Singosari Malang. Bahan dan peralatan yang digunakan diantaranya air ledeng, eosin-negrosin, mikroskop cahaya, hand tally counter, container mini 3 liter. Perlakuan yang diberikan antara lain : P1. Thawing pada air bersuhu 15oC berdurasi 30 detik dan P3. Thawing pada air bersuhu 37oC berdurasi 15 detik. Variabel yang diamati meliputi : motilitas individu, viabilitas dan abnormalitas. Data hasil penelitian yang berupa persentase ditransformasi ke arcus sinus kemudian di analisis menggunakan analisis ragam dengan Rancangan Acak Kelompok (RAK) dengan 10×ulangan sebagai Blok. Apabila hasil tersebut menunjukkan perbedaan, maka dilanjutkan dengan uji Beda Nyata Terkecil
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