PENGARUH KOMBINASI EKSTRAK JERUK BRASTAGI DAN WORTEL PER ORAL SEBELUM AKTIVITAS FISIK TERHADAP PENURUNAN KADAR MDA PLASMA MENCIT SETELAH AKTIVITAS FISIK

Abstract

Aerobic metabolism in the cellular level generates free radicals. Under normal condition,theres balance between free radicals and endogenous antioxidants. Excessive amount of freeradicals impair DNA, protein, fat, etc. The level of free radicals can be known by measuringplasma malondialdehyde level. Combination of Brastagis oranges and carrots juice asexogenous antioxidants supplementation expected to decrease free radicals level . The aim of thisstudy is to investigate the difference of plasma MDA level during several time intervals on micewhich is given and not given combination of Brastagis oranges and carrots juice before physicalactivity using mices treadmill for 20 minutes. The research method used in this study is anexperimental laboratory study. The objects of this study are 40 mice (mus musculus), whitecolored, male, weighting 25-30 grams, which is randomly chosen. The objects are divided into 2groups, Group A : 20 mice (given combination of Brastagis oranges and carrots juice beforephysical activity using mices treadmill) and group B : 20 mice (not given combination ofBrastagis oranges and carrots juice before physical activity using mices treadmill). Group Aare divided into 5 subgroups: A1 (measurement of plasma MDA level at 0 minute after treadmill),A2 (measurement of plasma MDA level at 15 minutes after treadmill), and A3 (measurement ofplasma MDA level at 30 minutes after treadmill), A4 (measurement of plasma MDA level at 60minutes after treadmill), and A5 (measurement of plasma MDA level at 240 minutes aftertreadmill). The same procedures are employed for the group B. Plasma MDA level measuredafter doing physical activity using mice treadmill. The homogenity of the result then was testedusing Levenes test and the normality of the result was tested using Kolmogorov-smirnov test (p>0.05). Further, the data was analyzed using independent t-test (p?0.05), one-way ANOVA(p?0.05) then Duncans test were used. The results reveal significant lowering plasma MDAconcentration in mice receiving combination of Brastagis oranges and carrots juice beforephysical activity, which is measured during several time intervals : 0,15,30,60, and 240 minutesafter physical activity than in mice not receiving combination of Brastagis oranges and carrotsjuice before physical activity. The MDA level differences between groups which is given and notgiven combination of Brastagis orange and carrots juice before physical activity measuredduring several intervals are 11,44% (0,8920 vs 1,0071) measured 0 minute after physical activity,15,47% (0,7902 vs 0,9348) measured 15 minutes after physical activity, 14,42% (0,7473 vs0,8732) measured 30 minutes after physical activity, 11,35% (0,6696 vs 0,7554) measured 60minutes after physical activity, and 13,60% (0,5786 vs 0,6696) measured 240 minutes afterphysical activity.The conclusion of the study suggested that combination of Brastagis orange andcarrots juice supplementation has lowering effect toward plasma MDA level measured duringseveral time intervals.