Pengaruh Hiperglikemia dan Vitamin E pada Kadar Malonaldehida dan Enzim Antioksidan Intrasel Jaringan Pankreas Tikus

Abstract

Prevention of pancreatic tissue damage by free radicals under conditions of hyperglycemia chronic would be important and vitamin E can act as scavengers of these oxygen radicals in preventing the development of diabetes mellitus. This study aims was to evaluate the effect of vitamin E on the hyperglycemia conditions on the blood glucose, enzyme superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels in rat pancreatic tissue. Research conducted at the Laboratory of Biochemistry Faculty of Veterinary Medicine Udayana University, in 2010. A total of 15 male rats white of strains Spraque Dawley old 3 months used in this study. All of rats were divided into three groups. Negative control group (K0), group of rats hyperglycemia (K1), and group of hyperglycemia and given vitamin E dose of 7 mg/200 g bw/day (K2). Hyperglycemia group was given 50% glucose solution doses of 1.5 cc/head orally twice daily. Treatment was conducted for 1 month. Blood glucose level was measured by using glucose oxidase biosensor method. Pancreatic SOD, GPx activities, and MDA levels were measured by using spectrophotometric methods. Blood glucose levels in the K2 treatment of 153.5±8.2 mg/dL was not significantly different (p>0.05) when compared with the K1 treatment of 154.1±5.7 mg/dL. Activities of SOD enzymes in the K2 treatment of 43.21±3.32 U/g higher than the K1 treatment of 31.99±3.55 U/g (p0.05) when compared with K1 treatment of 9.39±0.59 U/g. MDA level in the K2 treatment of 20.27±0.87 pmol/g lower than the K1 treatment of 25.24±0.82 pmol/g (p