Abstract

 
 
 
 Chlorophyll and carotenoids are antioxidant compounds that are very potential. Both of these compounds are found in many vegetables. This research aims to determine the levels of chlorophyll and carotenoids in mustard (Brassica) leaves. There were three types of mustard leaves tested, namely greens mustard (Brassica rapa I.), white mustard (Brassica juncea L.) and pakcoy (Brassica rapa L.). Analysis of samples by the Uv-Vis Spectrophotometry method. The results of the measurement of chlorophyll levels showed that pakcoy (Brassica rapa L.) had the highest chlorophyll level which was 86,76 mg/g. While the lowest chlorophyll level is found in the type of white mustard which is equal to 0,75 mg/g. From the three of the mustard tested, green mustard (Brassica rapa I.) and pakcoy (Brassica rapa L.) were mustard types that contained high carotenoids in the amount of 50,4 mg/g and 198,04 mg/g. While the type of white mustard (Brassica juncea L.) has the lowest carotenoid content for all preparations.
 
 
 
Highlights
Chlorophyll and carotenoids are antioxidant compounds that are very potential
Both of these compounds are found in many vegetables
This research aims to determine the levels of chlorophyll and carotenoids in mustard
Summary
Kimia Analisis Akademi Analis Farmasi Al – Islam Yogyakarta. Alat yang diperlukan yaitu: Spektrofotometri UV-Vis dan alat gelas laboratorium. Sedangkan bahan yang diperlukan: aseton 80 %, aquades, sampel sawi yang diperoleh dari Pasar Giwangan Yogyakarta berupa sawi hijau (Brassica rapa I.), sawi putih (Brassica juncea L.), dan pakcoy (Brassica rapa L.). Cara Kerja yang dilakukan adalah persiapan alat dan bahan, kemudian preparasi sampel. Preparasi sampel kering dilakukan dengan memotong sampel kemudian di jemur di bawah sinar matahari hingga mongering dan kadar airnya berkurang. Sebanyak 1 gram sampel dimaserasi selama 72 jam menggunakan aseton 80%. Setelah itu sampel basah dan kering diukur kadar klorofil serta karotenoidnya dengan Metode Spektrofotometri UV-Vis pada λ 663 ; 645 ; 480 nm. Kadar klorofil dan karotenoid dihitung dengan rumus berikut [7]: a.
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