Abstract
ABSTRACT 
 Tiger milk mushroom (Lignosus rhinocerus) is a plant that has traditionally been used as a medicine in the interior of West Kalimantan, especially in Kapuas Hulu. One of the phytochemical constituents contained is flavonoids (flavones and flavanones). This study aims to determine the total flavonoid content of ethanol extract of tiger milk mushroom. The simplicia of tiger milk mushroom was macerated using 96% ethanol. Phytochemical screening for the presence of flavonoids the extract using magnesium and amyl alcohol powder. Determination of total flavonoid content in ethanol extract of tiger milk mushrooms was carried out by UV-Vis spectrophotometry using the colorimetric method (AlCl?) at ? 410 nm and expressed as total flavonoids in quercetin equivalent. The results showed that total flavonoid content of ethanol extract is 33.041 mgEQ/g extract. Ethanol extract of tiger milk mushroom which contains flavonoids is potential as a source of natural antioxidants and potential to be developed in medicinal and cosmetic products.
 Keywords: Colorimetry, tiger milk mushroom, total flavonoid content.
Highlights
Jamur susu harimau (Lignosus rhinocerus) merupakan tanaman yang digunakan dalam pengobatan tradisional di pedalaman Kalimantan Barat terutama di Kapuas Hulu
This study aims to determine the total flavonoid content of ethanol extract of tiger milk mushroom
The results showed that total flavonoid content of ethanol extract is 33.041 mgEQ/g extract
Summary
Jamur Susu Harimau (Lignosus rhinocerus) merupakan tanaman tradisional yang berasal dari pedalaman Kalimantan Barat terutama di Kapuas Hulu. Menurut Tan et al (2009) bagian jamur kolorimetri digunakan untuk penetapan yang bermanfaat secara medis adalah kadar flavonoid yaitu dengan sklerotia. Peneltian sebelumnya menunjukkan adanya konstituen fitokimia seperti alkana, asam-asam lemak, benzena, penetapan kadar flavonoid total yang terkandung dalam ekstrak etanol jamur susu harimau (Lignosus rhinocerus). Bahan-bahan yang digunakan dalam penelitian ini yaitu jamur susu harimau (Lignosus rhinocerus) yang diperoleh dari Kabupaten Kapuas Hulu, Kalimantan Barat, etanol 96%, etanol pro analisis (Merck), asam asetat, asam klorida, serbuk magnesium, quersetin, dan AlCl3. Penentuan kadar flavonoid total pada ekstrak etanol jamur susu harimau dilakukan secara spektrofotometri UV-Vis dengan metode kolorimetri (AlCl3) pada λ 410 nm dan dinyatakan sebagai flavonoid total dalam ekuivalen kuersetin (EQ) (Rebaya et al, 2019)
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