PENENTUAN AKTIVITAS INULINASE PADA SUBSTRAT INULIN A1-KG DARI BAKTERI MESOFILIK RIZOSFER UMBI DAHLIA (DAHLIA SP)

Abstract

Inulinase is an enzyme that can catalyze inulin hydrolysis reaction to fructose and fructooligosaccharide (FOS) which two important compounds in the food industry. This study aims to determine the optimum activity of inulinase on variations in pH (3.5; 4; 4.5; 5; and 5.5), temperature (18oC; 25oC; 28oC; 37oC and 45oC); and substrate concentration (0.5%; 1%, 1.5%, 2% and 2.25%) with an incubation time of 30 minutes. In addition, this study also aims to determine the value of KM, Vmax and determine the type of action of inulinase. Determination of the optimum activity of inulinase was determined by DNS reagents whose absorbance was measured using spice at λ 536 nm. Determination of the type of action of the inulinase enzyme is determined by the TLC method. Inulinase activity found optimum at pH 4; 40oC temperature and 2% substrate concentration. The values of KM and Vmax inulinase are 3.12% (w / v) and Vmax is 0.288 U / mL. The type of action of inulinase enzymes found in Rhizosphere bacteria dahlia isolates A1-KG is endoinulinase. Keywords: inulin, inulinase, inulinase enzyme activity, and type of inulinase action from bacteria.