Penaeid shrimp cell culture and its applications

Abstract

AbstractThe aquaculture of penaeid shrimp has grown rapidly during the past three decades. However, shrimp aquaculture continues to be plagued by infectious disease, particularly of viral aetiology. Urgently needed are in vitro biological tools to facilitate early detection and diagnosis of penaeid shrimp viruses. An immediate need is the establishment of stable or continuous shrimp cell lines, which remains to be an elusive goal. Researchers have made significant advancements in the methodologies for primary shrimp cell culture. Also, new media and technologies including cell immortalization are presently under development. The principal goal of this review paper is to collate current attempts in the establishment of permanent shrimp cell lines by summarizing the latest research progress on penaeid shrimp cell culture studies, including general cultivation conditions, methods of cell preparation, types of media and their utilization and the development of immortal cell lines. In addition, current and potential applications of penaeid shrimp cell cultures in the various related research fields are also discussed in this review.