Abstract
Phycocyanin from Spirulina platensis (S. Platensis) is a pigment-complex protein belong to the light-harvesting phycobiliprotein family. The pigments have high economic value as a natural blue dye as well as the source of antioxidants and anticancer. Production of pure natural phycocyanin remains in high demand. Therefore, this study aimed to obtain phycocyanin with high purity values using modified liquid biphasic flotation (LBF) system and tested for DPPH (1,1-diphenyl-2-pycrilhydrazil) radical scavenging activity. This study produced high purity phycocyanin with purification fold 3.041 ± 0.04 and recovery yields approximately about 70.881%. Purified phycocyanin showed scavenging activity with IC50 of 338.585 mg/mL. Thus, the LBF system yielded high purity phycocyanin pigments.
Highlights
a pigment-complex protein belong to the light-harvesting phycobiliprotein family
Production of pure natural phycocyanin remains in high demand
& Supriyantini, E., Aktivitas anti oksidan fikosianin dari Spirulina Sp. menggunakan metode transfer elektron dengan DPPH (1, 1difenil-2-pikrilhidrazil)
Summary
Bahan kimia Bahan yang digunakan dalam penelitian ini yaitu biomassa Spirulina platensis yang diperoleh dari toko Fajar yang sudah bersertifikat Food and Drug Administration (FDA) United State (US), buffer fosfat pH 7 (K2HPO4 & KH2PO4) Merck, PEG 4000 g/L (Merck) dan K2HPO4 250 g/L (Merck), DPPH 0.1 mM (Aldrich), metanol p.a (Merck), vitamin C (Merck), NuPAGE Bis-Tris Gels 4% stacking & 12% running gel (Invitrogen), bolt LDS sample buffer (Invitrogen), bolt reducing agent (Invitrogen), bolt MES SDS running buffer (Invitrogen), commasie briliant blue (GBiosciences), asam asetat glasial (Fisher Scientific) dan akuades. Peralatan Alat-alat yang digunakan dalam penelitian ini adalah beberapa peralatan gelas, aluminum foil, autoklaf, timbangan analitik (Kern Abj), sentrifus (Health H-C-12 Centrifuge), pompa udara, corong filter kaca porositas Grade G4 (Quickfit), spektrofotometer UV-VIS (Thermo Scientific), gel cassette (Invitrogen), mini gel tank (Invitrogen), pipet mikro (Fraser), tips mikropipet 10 & 1000 μL (Kartell), vorteks (Wiggens), spin down, heating block (Corning), shaker (BJPX-103B), PowerEase power supply (Invitrogen), gel knife (Invitrogen)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
