Abstract
The research to determine the effect of fruit extracts juwet on the number and morphology of spermatozoa were superbly exposed to smoke has been conducted. This study uses rats aged 3 month, 200-210 gram, total 24 rats consisting of 4 treatment, is control, smoke of cigarette, juwet fruit extracts, and smoke of cigarette to juwet fruit extracts. Each treatment consisting 6 replications, each consisting of 1 rat. Before, acclimatization of rat treated 7 days. Way of giving juwet fruit extracts the gavage method 2 ml once daily for 48 days, while the CMC-Na 0,5% is given control. The exposure to cigarette smoke is given from an aerator pump , to given once daily for 48 days. Data were analyzed with ANOVA, if they were 5% significantly different would be followed by a DMRT. The result showed that the number of abnormal sperm morphology were significantly diferent (p<0,05). The averange number of abnormal spermatozoa K1 group was higher than K3 group. The number of spermatozoa was not significant different between treatments. The average number of spermatozoa K1 lower than K3. This is because the smoke of cigarette increased formation of ROS and the resulting stress oxidative and cause cell damage tissues and organs, especially to the reproductive system. Juwet fruits extract have been able to improve the count and morphologyof rats spermatozoa expose to smoke. This case because of antioxidant from juwet fruit can be protect cell from the free radical attack.
 Keywords: Rattus sp, smoke of cigarette, java plum fruit, spermatozoa, , ROS, antioxidant.
Highlights
The research to determine the effect of fruit extracts juwet on the number and morphology of spermatozoa were superbly exposed to smoke has been conducted
24 rats consisting of 4 treatment
each consisting of 1 rat
Summary
Sejumlah 24 ekor tikus putih jantan berusia sekitar 3 bulan dengan berat 200-210 gram digunakan sebagai hewan coba. K1 adalah kelompok tikus yang diberi paparan asap rokok. K3 adalah kelompok tikus yang diberi paparan asap rokok dan ekstrak buah juwet. Berdasarkan analisis dengan uji One Way ANOVA, nilai rata-rata morfologi spermatozoa abnormal pada kelompok K1 berbeda nyata dengan kelompok K3. Nilai rata-rata morfologi spermatozoa abnormalK2berbeda nyata dengan kelompok K0 dan K1. Sedangkan nilai rata-rata morfologi spermatozoa normal pada kelompok K0 berbeda nyata antar perlakuan. Nilai rata-rata morfologi spermatozoa normal pada kelompok K2 berbeda nyata dengan kelompok K0 dan K1. Hasil rataan dan standar deviasi morfologi dan jumlah spermatozoa tikus putih jantan (Rattus sp.) pada kelompok kontrol dan perlakuan setelah pemberian paparan asap rokok dan ekstrak buah juwet (Syzygium cumini L.) selama 48 hari
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