Abstract

Background: Pumpkin (Curcubita moschata Durch) is a type of vegetable that can be easily damaged like cracked or scratched as the harvesting or post-harvesting time. Those imperfect pumpkins will be rotten in a short time so that they cannot be sold in the market. Therefore, they need to be processed into a durable functional food in the form of cookie; the pumpkin is as the main ingredient for the dough and Lactobacillus sp, the probiotics bacteria is added into it. Research purposes: The aim is to find out the exact concentration of the coating materials’ combination and find out the viability of probiotics which is encapsulated in the maltosedextrin. Method: The coating materials’ combination is examined through encapsulation process using 10 % skimmed milk with three different precentage of maltodextrin i.e. 10%, 15% and 20%. The viability test was carried out using the spread plate method and then analyzed using the Colony Forming Unit and Viability (%) calculation method. Results and Discussion: The coating materials’ combination at concentration of 10% skimmed milk + 10%, 15% and 20% maltodextrin produce 84%, 90% and 98% cells’ viability. This shows that the use of those combinations can maintain the viability of the bacteria from the heat during the baking process at 180°C for 13 minutes. Conclusion: The microencapsulation of Lactobacillus achidopilus and Bifidobacterium logum with 10% skimmed milk + 20% maltodextrin resulted in the best viability as much as 98% viability cells. The higher the maltodextrin amount, the greater the bacterial’s viability is.

Highlights

  • Pumpkin (Curcubita moschata Durch) is a type of vegetable that can be damaged like cracked or scratched as the harvesting or post-harvesting time

  • Those imperfect pumpkins will be rotten in a short time

  • so that they cannot be sold in the market

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Summary

Wardani dan Dewi

Volume 12, No.[2], (2021), Halaman [239-249] metode spread plate dan dianalisis dengan metode perhitungan CFU (Coloni Forming Unit) dan Viabilitas (%). Hal ini menunjukkan bahwa penggunaan kombinasi bahan penyalut tersebut dapat mempertahankan viabilitas bakteri dari panas akibat proses pemanggangan dengan oven pada suhu 180°C selama 13 menit. Hal ini menunjukkan bahwa semakin tinggi penambahan maltodekstrin yang dikombinasikan dengan susu skim 10%, menghasilkan viabilitas sel bakteri yang tinggi. Penelitian ini ingin memanfaatkan probiotik dalam cookies labu kuning sebagai inovasi pangan fungsional yang memanfaatkan komoditas lokal. Samalukang, 2017) dan mampu meningkatkan viabilitas bakteri pada pH rendah (Samedi & Charles, 2019) sehingga diperkirakan dapat mempertahankan viabilitas probiotik apabila diaplikasikan dalam cookies labu yang memerlukan pemanasan. Penelitian ini bertujuan untuk mengetahui konsentrasi kombinasi bahan penyalut yang baik untuk pembuatan produk cookies probiotik dan untuk mengetahui viabilitas probiotik yang terenkapsulasi maltosedekstrin dalam cookies labu kuning. Analisis data Pada penelitian ini data di uji menggunakan Uji One Way Anova pada SPSS 16, untuk mengetahui apakah terdapat perbedaan rata-rata viabilitas bakteri pada masing-masing perlakuan

HASIL DAN PEMBAHASAN
Pada pengkulturan murni bakteri
Waktu inkubasi
Pada proses enkapsulasi bakteri
Analisis data menggunakan uji one way ANOVA
Ubi Kayu Dengan Tepung Labu
Fungsi Maltoedekstrin dari Pati
Fungsional dari Tanaman Lokal
Penyalut Terhadap Viabilitas dan Karakteristik
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