Abstract
The dye functions to clarify the plant tissue that will be observed with a microscope, the dye that is commonly used is synthetic dyes that are carcinogenic, the price is expensive and can pollute the environment. Natural dyes are an alternative to synthetic dyes because they are safe, inexpensive and environmentally friendly. natural dye sources obtained from plant parts such as fruit peels. Efforts to use mangosteen rind waste as an herbal remedy can also be used as a natural dye because it has a high enough anthocyanin content. Anthocyanins in mangosteen peel can be obtained through extraction. Maceration is an easy extraction method where the results are only affected by the type of solvent and the extraction time. Purslane (Portulaca oleracea L.) is used as research material because currently purslane has been widely used because of its nutritional content including high metabolic and antioxidant regualting substances. This study aims to determine the ability of mangosteen rind extract in coloring the leaf and stem tissue of purslane, and to determine the type of solvent and optimal immersion time to produce mangosteen rind extract so that it can color the leaf tissue and stems purslane well. The study was conducted experimentally using a Completely Randomized Design (CRD) with 2 factors: factor 1 was the type of solvent using ethanol 96% and citric acid 14%, the second factor was immersion time, namely 26, 27 and 28 hours. The data obtained were analyzed descriptively qualitatively including the contrast and clarity of the preserved tissue preparation of leaf and stem purslane. The results showed mangosteen rind extract has the ability to dye leaf tissue and stems. The type of 14% citric acid solvent with 28 hours soaking time was optimal in producing mangosteen rind extract so that it was able to dye the leaf and stem tissue of purslane.
Highlights
Abstrak Pewarna alami menjadi alternatif pengganti pewarna sintetis dikarenakan bersifat aman, murah dan ramah lingkungan
This study aims to determine the ability of mangosteen rind extract in coloring the leaf and stem tissue of purslane, and to determine the type of solvent and optimal immersion time to produce mangosteen rind extract so that it can color the leaf tissue and stems purslane well
The results showed mangosteen rind extract has the ability to dye leaf tissue and stems
Summary
Bahan yang digunakan pada penelitian ini adalah kulit buah manggis, daun dan batang krokot, etanol 96%, asam sitrat 14%, larutan fiksatif FAA, alkohol 70%, alkohol 80% dan alkohol 96%, etanol PA, xylol, parafin murni, akuades, gliserin, albumin dan entelan, sedangkan alat yang digunakan pada penelitian ini adalah blender, alumunium foil, batang pengaduk, saringan, silet, botol flakon, oven, cutter, holder, object glass, thermostatstaining jar, rotary microtom, cover glass, mikroskop, gelas arloji, beaker glass, gelas ukur, pipet tetes, pinset, kamera, alat tulis, kertas label, baki, bunsen dan tisu. Variabel terikat yaitu jaringan daun dan batang krokot yang terwarnai oleh ekstrak kulit buah manggis. Parameter yang diamati yaitu kekontrasan warna dan kejelasan preparat daun dan batang krokot. Pembuatan preparat awetan irisan melintang daun krokot dilakukan dengan menggunakan metode embedding yang dimodifikasi (Sass, 1951). Preparat dibuat 3 macam : preparat dengan pewarna ekstrak kulit manggis, preparat dengan pewarna safranin sebagai pembanding, dan preparat tanpa pewarna sebagai kontrol. Pembuatan preparat awetan irisan melintang batang krokot dilakukan dengan menggunakan metode non-embedding yang dimodifikasi (Preece, 1959). Pengamatan Hasil Penelitian Kekontrasan dan kejelasan preparat diamati menggunakan mikroskop perbesaran 40x dan 100x, kemudian dibandingkan antara preparat yang diwarnai dengan pewarna alami ekstrak kulit buah manggis, preparat yang diwarnai dengan safranin dan preparat tanpa pewarnaan sebagai kontrol. Analisis Data Data dianalisis secara deskriptif kualitatif meliputi kekontrasan dan kejelasan preparat berdasarkan indikator kekontrasan dan kejelasan preparat
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