Pemanfaatan casa dalam observasi motilitas spermatozoa semen cair Sapi Madura dalam pengencer berbeda

Abstract

The purpose of this study was to measure the motility of Madura bull spermatozoa using three different diluents (tris aminomethane, CEP-2 and skim milk) duringcold storage. The research material used were 2 Madura bulls. Collecting semen method used artificial vagina, followed by fresh semen analysis and processing of liquid semen. Observation of liquid semen quality was carried out up to the 5th days of cold storage. Motility examination of semen liquid used Computerized Assisted Semen Analysis (CASA), namely SCA v.5.2. The parameters measured were: progressive motility, motility, velocity straight linear (VSL), velocity curve linear (VCL), velocity average pathway (VAP), straightness (STR), linearity (LIN), wobble (WOB), hyperactivity (H), Beat cross frequency (BCF), amplitude lateral head (ALH). Data was analyzed by Minitab 17. Motility and progressive motility of spermatozoa in tris aminomethane and CEP-2 were higher than skim milk for 5 days of cold storage. The VCL value of tris aminomethane was higher than CEP-2 and skim milk. VSL scores barely show the difference among diluents. VAP values in tris aminomethaneand CEP-2 are higher than skim milk at the beginning of storage. The LIN, STR and WOB values of the CEP-2 and skim milk yield higher values than tris aminomethane. The percentage value of hyperactivity spermatozoa was same among diluents. The ALH value of trisaminomethane was higher than CEP-2 and skim milk. BCF values in CEP-2 was higher than skim milk and tris aminomethane. Tris aminomethane and CEP-2 can support the motility of Madura bull spermatozoa than skim milkfor 5 days of storage