Abstract
Hepatic ischemia-reperfusion injury (HIRI) is a common phenomenon in liver transplantation and liver surgery. This article is aimed at clarifying the role of pemafibrate in HIRI through JAK2/STAT3β/PPARα. In the experiment, we divided Balb/c into seven groups, namely, normal control (NC), Sham, PEM (1.0 mg/kg), IRI, IRI + PEM (0.1 mg/kg), IRI + PEM (0.5 mg/kg), and IRI + PEM (1.0 mg/kg). We used biochemical assay, histopathological evaluation, immunohistochemistry, RT-PCR and qRT-PCR, ELISA analysis, and other methods to determine the level of serum AST, ALT, IL-1β, and TNF-α in the liver at three time points (2 h, 8 h, and 24 h) after reperfusion of apoptosis factor, autophagy factor, and the JAK2/STAT3/PPARα content in tissues. Our experiment results showed that the pemafibrate can effectively reduce the level of hepatic IR injury. In addition, pemafibrate has anti-inflammatory, antiapoptotic, and antiautophagy effects, which are mediated by the JAK2/STAT3β/PPARα pathway.
Highlights
Hepatic ischemia-reperfusion injury (HIRI) is the injury caused by reperfusion after liver ischemia [1, 2]
After the blood supply to liver tissue was interrupted due to liver ischemia, the subsequent blood reperfusion brings in a large number of inflammatory cells, which leads to serious damages to the structure and function of the liver [3,4,5]
Observation on the mouse liver tissues with hematoxylin and eosin (H&E) staining found that the structure and function of the liver tissues were not remarkably changed in H&E-stained sections (Figure 1(b))
Summary
Hepatic ischemia-reperfusion injury (HIRI) is the injury caused by reperfusion after liver ischemia [1, 2]. After the blood supply to liver tissue was interrupted due to liver ischemia, the subsequent blood reperfusion brings in a large number of inflammatory cells, which leads to serious damages to the structure and function of the liver [3,4,5]. ROS is the starting point that causes a cascade of reactions dominated by inflammatory cells, cytokine release, apoptosis, and autophagy [9, 10]. This damage affects the liver and has serious negative effects on the brain, heart, kidneys, and gastrointestinal tract, which is a complex systemic process
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